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Antisera

Colleague:

I am pleased that you are interested in using the antisera and/or cDNA probes that I make available to researchers. Please check the cross-reactivity panel for antisera at on the Reagents page to see if your choice of antisera is appropriate for your model system (although the list is not always complete so I will sometimes make changes or additions to your requests). Generally I do not send more than one antiserum against any one protein unless there is a clear reason for such duplicate requests. Unless otherwise noted, all antisera are w​hole rabbit serum. All antisera are made in limited amounts and therefore some may no longer be available.  Most antisera are whole rabbit serum antisera but there are a limited number of monoclonals. At this time, I usually cannot send monoclonal antibodies to foreign destinations due to high handling costs.

Santa Cruz Biotechnologies Inc. is now carrying our most commonly requested monoclonals.

 BSP (LFMb-24)  sc-73634  mouse IgG1  131-442 (h) WB   h, monkey
 BSP (LFMb-25)  sc-73630  mouse IgG1  257-276 (h)  WB, IF, IHC(P)  h, monkey, cow
 DMP-1 (LFMb-31)  sc-73633  mouse IgG1  internal (h)  WB, IF, IHC(P)  h, monkey
 DSP (LFMb-21)  sc-73632  mouse IgG1  487-502 (h)  WB, IF, IHC(P)  h, monkey
 OPN (LFMb-14)  sc-73631  mouse IgG2b  173-186 (h)  WB, IF, IHC(P)  h, monkey, mou (not WB), rab, rat, sheep
 MEPE (LFMb-33)  sc-73635  mouse IgG1  244-262 (h)  WB, IF, IHC(P)  h, monkey

For most monoclonals, animal species other than human, monkey & mouse have not been tested.

To comply with the National Institutes of Health’s requirements I will generate a Transfer Memo using the information you send to me.  After the NIH has assigned a tracking number to the request document I will forward it to you.  You will complete the document and email the completed form to NIDCReMTA@mail.nih.gov. Contact Dr. David Bradley, bradleyda@nidcr.nih.gov, if you have any questions about the official form.  (I can usually answer questions about the reagents themselves, but NIH controls the procedures about releasing them to you.)

When sending your initial information to me, please note a very brief research project (1-3 sentences) including the model species (mouse, cow, human etc.) and techniques you plan to use (paraffin sections, Western blot etc.) so that I can send the most appropriate antisera/cDNA. I will send the Research Materials after NIH has accepted the completed requests and instructed me to send the materials. I reserve the right to limit the number probes sent (particularly in the case of duplicate requests of antisera for detecting the same protein) and to substitute in cases where antisera are limited or I think that a different probe would be more effective. Please remember that our web site is not often updated. My apologies for any errors or confusion.

For colleagues in COMMERCIAL laboratories, you MUST contact the Technology Development Coordinator here at the NIDCR (Dr. David Bradley, bradleyda@nidcr.nih.gov) about the necessity of obtaining a license for the probes. Questions about legal or technical aspects of the SLA should be directed to Dr. Bradley.


Upon receipt of the signed SLA from NIH, I will ship without charge by regular airmail. For requests from within the USA, inclusion of your FedEx number (and phone number) may shorten the shipping times at your expense.

Sincerely,

Larry W. Fisher, PhD
Chief, Matrix Biochemistry Section
Craniofacial and Skeletal Diseases Branch
NIDCR/NIH

The list of Antisera are shown in the following tables:

 

 

Proteoglycan polyclonal antisera

 

Gene product

Antiserum Name

Antigen source

Known species

Comments

References

Human decorin

LF-136

GIGPEVPDDRDF-[KLH]

Hum & Mon only

increased signal strength with pretreatment with ABC'ase

Fisher et al. 1989b

Bianco et al. 1990

Human decorin propeptide

LF-110

QVSWAGPFQQRGLFDC-[LPH]

Hum, others not determined but seq

no cross to BGN propeptide

Fisher et al.
1995

Human decorin propeptide

LF-111

QVSWAGPFQQRGLFDC-[CSA] (amino acids 5-17)

Hum, others not determined but seq is highly conserved

no cross to BGN propeptide

Fisher et al.
1995

Human decorin

LF-122

recombinant protein including propeptide

Hum, Mon, Pig, She, Chi, cow(?)

 

Fisher et al.
1995

Human biglycan

LF-51

GVLDPDSVTPTYSA-[BSA] (amino acids 11-24) same conjugate for all three antisera

Hum & Mon only

increased signal strength with pretreatment with ABC'ase

Fisher et al. 1989b

Bianco et al. 1990

Human biglycan

LF-112

GVLDPDSVTPTYSA-[BSA] (amino acids 11-24) same conjugate for all three antisera

Hum & Mon only

increased signal strength with pretreatment with ABC'ase

Fisher et al. 1989b

Bianco et al. 1990

Human biglycan propeptide

LF-104

LPFEQRGFWDFTLDDC-[LPH]

Hum, Mon, Rat, Mou

 

Fisher et al. 1995

Human biglycan propeptide

LF-105

LPFEQRGFWDFTLDDC-[CSA]

others not determined

 

Fisher et al. 1995

Human veriscan

LF-99

LHKVGKSPPVRC-[LPH]

only human tested

 

Bernstein et al. 1995b

Human veriscan

LF-98

LHKVGKSPPVRC-[CSA]

 

 

 

Human biglycan

LF-121

recombinant protein including propeptide

Hum & Mon only

 

Fisher et al. 1995

Bovine decorin

LF-94

IGPEEHFPEVPEC-[LPH]

only cow tested

Cow domain analogous to Human LF-30

Fisher et al. 1995

Bovine decorin

LF-95

IGPEEHFPEVPEC-[CSA]

for these 2 antisera

Cow domain analogous to Human LF-30

Fisher et al. 1995

Bovine biglycan

LF-96

LPDLDSLPPTYSC-[LPH]

only cow tested

Cow domain analogous to Human LF-15 series

Fisher et al. 1995

Bovine biglycan

LF-97

LPDLDSLPPTYSC-[CSA]

for these 2 antisera

Cow domain analogous to Human LF-15 series

Fisher et al. 1995

Murine decorin

LF-113

IIPYDPDNPLISMC-[LPH]

only tested for

Mouse domain analogous to Human LF-30 series

Fisher et al.
1995

Murine decorin

LF-114

IIPYDPDNPLISMC-[CSA]

Mou and Rat

Mouse domain analogous to Human LF-30 series

Fisher et al.
1995

Murine biglycan

LF-106

VPDLDSVTPTFSAMS-[LPH]

only tested for

Mouse domain analogous to Human LF-15 series

Fisher et al.
1995

Murine biglycan

LF-107

VPDLDSVTPTFSAMS-[CSA]

Mou and Rat

Mouse domain analogous to Human LF-15 series

Fisher et al.
1995

Murine biglycan

LF-159

VPDLDSVTPTFSAMS-(LPH)

Mou and Rat

Mouse domain analogous to Human LF-15 series

 

Murine fibromodulin

LF-149

CDKVGRKVFSKLRHLER & CDPYDPYPYEPSEPYPYGVEE conjugated to KLH

Mouse, others not tested

histology requires digestion with chondrointinase ABC

 

Murine fibromodulin

LF-150

CDKVGRKVFSKLRHLER & CDPYDPYPYEPSEPYPYGVEE conjugated to KLH

Mouse, others not tested

histology requires digestion with chondrointinase ABC, same as LF-149

 



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Sialoprotein polyclonal antisera

 

Gene Product

Antiserum name

Antigen source


Known species cross-reactivity

Comments

References

Human osteopontin

LF-19

IPVKQADSGE homopolymer

Hum & Mon only

 

Fisher et al. 1989b

Human osteopontin

LF-86

TVDTYDGRGDSVVYGLR
SLS-[LPH]

Hum, Mon

human osteopontin RGD domain unknown utility

Fisher et al.
1995

Human osteopontin

LF-161

CLYNKYPDAVATWLNPDPSQKQNLLAPN conjugated to KLH

only human tested

human exon 4

 

Human osteopontin

LF-166

recomb. Carboxyl half starts at thrombin site

Hum, Mon, Pig, Cow, She, Rat, Mou

does not contain the RGD domain

Fisher et al. 1995 based onOP-10 plasmid (Young et al. 1990a)

Human osteopontin

LF-124

recombinant amino half ending at thrombin site

Hum, Mon, Cow, She, Rat, Mou

contains the RGD domain

Fisher et al. 1995 based on OP-10 plasmid (Young et al. 1990a)

Mouse OPN

LF-175

recombinant mouse OPN (E coli)

Mou, Rat

OK for WB, IF, IHC(P)

 

Human osteopontin

LFMb-14 Monoclonal*, IgG2b

human osteopontin, likely epitope is within KFRRPDIQYPDATD

All tested to date: Hum, Mou, Rat, Sheep, Rabbit, Monkey

Mouse monoclonal (IgG) works in westerns and paraffin sections

 

Human bone sialoprotein

LFMb-24 Monoclonal*, IgG1

recombinant (AA131-442)

Hum, Monkey

May be slightly more sensitive on WB than LFMb-25

Bellahcène A, Castronovo V, Ogbureke KU, Fisher LW, Fedarko NS. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.

Human bone sialoprotein

LFMb-25 Monoclonal*, IgG1

recombinant (AA131-442), epitope probably within: VAYEGEYEYTGANGYDNGYE

Hum, Monkey

OK for WB, IF & IHC(P)

Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.

Human bone sialoprotein

LF-83

YESENGEPRGDNYRAYE

D-[LPH]

Hum, Mon, Dog

epitope appears to be RAYED

Bianco et al. 1993

Mintz et al. 1993

Human bone sialoprotein

LF-84

YESENGEPRGDNYRAYE

D-[LPH]

Rat, Mou, Chi

 

Fisher et al.
1995

Human bone sialoprotein

LF-100

AIQLPKKAGDIC-[LPH]

Hum, Mon, Dog, Rat

mid-protein antigen

Mintz et al. 1993

Human bone sialoprotein

LF-101

AIQLPKKAGDIC-[CSA]

Hum, Mon, Dog, Rat, Pig, Cow

mid-protein antigen

Fisher et al.
1995

Human bone sialoprotein

LF-119

recombinant RGD domain (AA257-317)

Hum, Mon, Dog, Chi

mid-protein antigen similar to LF-100

Fisher et al.
1995
based on B6-5g plasmid (Fisher et al. 1990)

Human bone sialoprotein

LF-120

recombinant Frag 1 (aa 129-281)

Hum, Mon, Pig, Dog, Rat

 

Fisher et al.
1995
based on B6-5g plasmid (Fisher et al. 1990)

Rat (UMR) bone sialoprotein

LF-87

rat BSP isolated from UMR-106-BSP media

Rat, Mou, Hom

full sulfated form of rat BSP

Fisher et al.
1995

Bianco et al. 1993

Rat (UMR) bone sialoprotein

LF-90

rat UMR-106 BSP grown in chlorate

Rat

Low sulfate(<5%) sulfate 0n BSP

Fisher et al.
1995

Human dentin matrix protein-1 (DMP1)

LFMb-31

recombinant exon 5 (last), epitiope probably within: SESQEEVVSESRGDNPDPTTSYVE

Hum, Monkey

OK for WB (but DMP1 is difficult to capture in standard WB) IF, IHC(P)

Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.

Human dentin matrix protein 1 (DMP1)

LF-148

CEHPSRKIFRKSRISE and CLKNIEIESRKLTVDAYH conjugated to KLH

human and bovine, others not tested

 

 

Human dentin matrix protein 1 (DMP1)

LF-160

CGSDDHQYIYRLAGGFSRSTGKGGDD & CRDTGLSQPRRDSKGDSQED conjugated to KLH

human and bovine, others not tested

 

 

Human DSP (of DSPP)

LFMb-21 Monoclonal*, IgG1

synthetic peptide: KGQEGQDHGKEDDHDS

Hum, Monkey

OK for WB, IF, IHC(P)

Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.

Hum dentin sialophosphoprotein (DSPP)

LF-151

part of DSP portion of human DSPP, bacterial recombinant

human and bovine, not mouse

 

 

Hum dentin sialophosphoprotein (DSPP)

LF-154

non repetitive portion of last exon, human DSPP

human and mouse, others not tested

appears to recognize DSP portion of last exon

 

Mou dentin sialophosphoprotein (DSPP)

LF-153

part of DSP portion of mouse DSPP, bacterial recombinant

mouse, not human or cow

 

 

Human MEPE

LFMb-31 Monoclona*, IgG1

recombinant last exon, epitope within THRIQHNIDYLKHLSKVKKI

Hum, Monkey

OK for WB, IF, IHC(P)

Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70. Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.

Human MEPE

LF-155

last exon of human MEPE made in bacteria

human, others not tested

 

 

Human MEPE

LF-158

CSIYPKSTGNKGFEDGDD & CSTHRIQHNIDYLKHLSKVKK conjugated to KHH

human, others not tested

weaker titer than LF-155 on ELISA

 



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Collagen polyclonal antisera

 

Gene product

Antiserum Name

Antigen source

Known Species Cross-reactivity

Comments

References

Bovine a1 (I) amino-propepetide

LF-9

bovine bone N-propeptide

Hum, Mon, Pig, She

 

Fisher et al. 1987b

Human a1 (I) amino-propepetide

LF-39

EEGQVEGQDEDIPPITCVQ-[KLH]

Hum, Cow, She

 

Fisher et al. 1989a

Human a1 (I) amino-propepetide

LF-40

EEGQVEGQDEDIPPITCVQ-[KLH]

Hum, Cow, She

 

Fisher et al. 1989a

Human a1 (I) carboxy-propept.

LF-41

PLDVGAPDQEFGFDVGPVCFL-[KLH]

All species except, perhaps Rab

detects proal(1) and C-propeptide

Fisher et al. 1989a

Human a1 (I) carboxy-propept.

LF-42

PLDVGAPDQEFGFDVGPVCFL-[KLH]

 

 

Fisher et al.
1995

Human a1 (I) carboxy-telopept.

LF-67

SAGFDFSFLPQPPQEKAHDGGRYYRA

All species except,

detects proal(1)

Bernstein et al. 1995b

Human a1 (I) carboxy-telopept.

LF-68

SAGFDFSFLPQPPQEKAHDGGRYYRA

perhaps Rab

pCal(1), a(1) + / - reduction

Fisher et al.
1995

Human a2 (I) amino-telopeptide

LF-116

QYDGKGVGLGPGPC-[LPH]

Hum, not mouse, others not tested

 

 

Human type III collagen C-propeptide

LF-69

DIGGPDQEFGVDVGPVCFL

Hum, Mon, others not tested

seq. homology to type II but not tested. Reduction increases reaction

Bernstein et al. 1995b

Human type III collagen C-propeptide

LF-70

DIGGPDQEFGVDVGPVCFL

Hum, Mon, others not tested

seq. homology to type II but not tested. Reduction increases reaction

Fisher et al.
1995

Human type III collagen N-telopeptide

LF-71

EYDSYDVKSGVAC-[KLH]

Hum, Mon,

 

Bernstein et al. 1995b

Human type III collagen N-telopeptide

LF-72

EYDSYDVKSGVAC-[KLH]

others not tested

 

Fisher et al.
1995



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Miscellaneous polyclonal antisera

Gene product

Antiserum Name

Antigen Source

Known species cross-reactivity

Comments

References

Human osteonectin

LF-37

EALPDETEVVEETVAEVT
EVP-[KLH] (AA 4-25)

Hum, Mon

Also known as SPARC

Fisher et al.
1995

Bovine osteonectin

BON-I

BON-II

bovine bone osteonectin (both)

All species tested

Also known as SPARC

Ingram et al. 1993

Bovine osteonectin

LF-55

LF-56

first 56 AA of mature Cow sequence (both)

only Cow and Hum tested

Also known as SPARC

Fisher et al.
1995

Bovine osteonectin

LF-53

LF-54

AA 27-56 of mature Cow sequence (both)

only Cow and Hum tested

Also known as SPARC

Fisher et al.
1995

Chicken osteonectin

LF-8

chicken bone osteonectin

Chi, Quail

Also known as SPARC

Fisher et al.
1995

Chicken osteonectin

LF-45

APQEALPDETEVIED-[KLH]

Chi, Quail

AA 1-15 of mature

Fisher et al.
1995

Mouse osteonectin

LF-23

LF-24

TEVAEEIVE EET-[KLH] (both) (AA 5-16 of mature)

Mou, Rat, She, Chi

Also known as SPARC

Wewer et al. 1988

Bovine osteocalcin

LF-32

bovine bone osteocalcin

Cow, Hum, Mon

also known as bone gla protein (BGP)

Ingram et al. 1993

Bovine alkaline phosphatase

LF-52

from bovine kidney affinity purified by Sigma

unknown

bone-liver-kidney form of the enzyme

Fisher et al.
1995

Human alkaline phosphatase

LF-47

LF-48

LVPEKEKDPKYWRDQAQ
ETC-[KLH] (both) (AA 1-19)

Hum (weak), Cow, Pig, Rab

human sequence but weak activity

Tulli et al. 1992

Human osteoinductive factor (OIF)

LF-102

LF-103

CLKRLPIGSYF-(LPH)

CLKRLPIGSYF-(CSA)

both not tested

C-term of OIF, both uncharacterized

Fisher et al.
1995

Human MMP-3

LF-182

synthetic peptide: EPGFPKQIAEDFPGIDSKIDAC

Hum, Monkey

IHC(P), WB probably OK

Ogbureke KU, Fisher LW.
SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem.
  2007 Apr;55(4):403-9. Epub 2007
Jan 8.

Human MMP-2

LF-183

synthetic peptide: ENQSLKSVKFGSIKSDWLGC

Hum, Monkey

IHC(P), WB probably OK

Ogbureke KU, Fisher LW.
SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem.
2007 Apr;55(4):403-9. Epub 2007
Jan 8.

Human MMP-9

LF-184

synthetic peptide: RSELNQVDQVGYVTYDILQCPED

Hum, Monkey

IHC(P), WB probably OK

Ogbureke KU, Fisher LW.
SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem.
2007 Apr;55(4):403-9. Epub 2007
Jan 8.

Mouse MMP-14

LF-176

catalytic domain synthetic peptide: CPLRFREVPYAYIREGHEKQAD

Hum, Mouse

IHC, WB

 

Mouse MMP-14

LF-177

hemopex domain synthetic peptide: CRTTSRPSVPDKPKNPAYG

Hum, Mouse

IHC(P), WB

 

Mouse MMP-14

LF-178

combined peptides from LF-176 & LF-177

Hum, Mouse

IHC(P), WB

 

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This page last updated: May 28, 2014