Intracellular Membrane Trafficking Unit
NATIONAL INSTITUTES OF HEALTH/NIDCR
BUILDING 30 ROOM 303A
30 CONVENT DRIVE MSC 4340
BETHESDA, MD 20892-4340
Phone: (301) 496-9969
Fax:(301) 496-1966
Cell: (301) 803-8129
E-mail:Dr. Roberto Weigert
Research interest
Our main interest is in membrane traffic. By transitioning from cell culture models to three dimensional systems and live organisms using intra-vital microscopy, we aim at elucidating the molecular mechanisms regulating both exocytic and endocytic events in mammalian cells.
Education
2000 Ph.D. Open University, London, UK, and Consorzio Mario Negri Sud, Italy
1992 M.Sc. Chemistry, University of Catania, Catania, Italy
Selected Publications
Lee SB, Park JH, Kaevel J, Sramkova M, Weigert R, Park MH. The effect of hypusine modification on the intracellular localization of eIF5A (2009) Biochem Biophys Res Commun. Jun 12;383(4):497-502.
Eyster CA, Higginson JD, Huebner R, Porat-Shliom N, Weigert R, Wu WW, Shen RF, Donaldson JG. Discovery of New Cargo Proteins that Enter Cells through Clathrin-Independent Endocytosis (2009)Traffic Mar 17. [Epub ahead of print]
Gavard J, Hou X, Qu Y, Masedunskas A, Martin D, Weigert R, Li X, Gutkind JS. A role for a CXCR2/phosphatidylinositol 3-kinase gamma signaling axis in acute and chronic vascular permeability (2009) Mol Cell Biol May;29(9):2469-80
Bhirde AA, Patel V, Gavard J, Zhang G, Sousa AA, Masedunskas A, Leapman RD, Weigert R, Gutkind JS, Rusling JF. Targeted killing of cancer cells in vivo and in vitro with EGF-directed carbon nanotube-based drug delivery (2009) ACS Nano Feb 24;3(2):307-16
Masedunskas A, Weigert R. Intravital two-photon microscopy for studying the uptake and trafficking of fluorescently conjugated molecules in live rodents. (2008) Traffic Sep;9(10):1801-10
Weigert R. and Donaldson JG. Fluorescent microscopy-based assays to study the role of Rab22a in clathrin-independent endocytosis (2005) Methods in Enzymology 403:243-253
Donaldson JG., Honda A., and Weigert R. Multiple Activities for Arf1 at the Golgi Complex (2005) Biochim Biophys Acta 1744(3):364-73
Weigert R., Yeung AC., Li J., and Donaldson J.G. Rab22a Regulates the Recycling of Membrane Proteins Internalized Independently of Clathrin. (2004) – Mol. Biol. Cell 15(8), 3758-3770
Naslavsky N.*, Weigert R*. and Donaldson J.G Characterization of a Non-Clathrin Endocytic Pathway: Membrane Cargo and Lipid Requirements. (2004) – Mol. Biol. Cell 15(8), 3542-3552
* Equal contribution
Altan-Bonnet N., Phair R.D., Polishchuk R.S., Weigert R, and Lippincott-Schwartz J. Arf1 inactivation is necessary for mitotic Golgi disassembly, chromosome segregation and cytokinesis. (2003) Proc Natl Acad Sci U S A. 100(23):13314-9
Naslavsky N., Weigert R. and Donaldson J.G. Convergence of non-clathrin and clathrin-derived endosomes requires ARF6 inactivation and phosphoinositide turnover. (2003) – Mol. Biol. Cell 14(2):417-431.
Weigert R., Silletta M. G., Spanò S., Turacchio G. , Cericola C., Colanzi A., Mancini R., Polishchuk E.V., Salmona M., Facchiano F., Burger K.N.J., Mironov A., Luini A., and Corda D. (1999). CtBP/Bars induces fission of Golgi membranes by acylating lysophosphatidic acid. Nature 402: 429-433.
Silletta M.G., Colanzi A., Weigert R., Di Girolamo M., Santone I., Fiucci G., Mironov A., De Matteis M. A., Luini A. and Corda D. (1999). Role of the brefeldin A-dependent ADP-ribosylation in the control of intracellular membrane transport. Mol. Cell. Biochem. 193: 43-51.
Mironov A., Colanzi A., Silletta M. G., Fiucci G., Flati S., Fusella A., Polishchuk R., Mironov A. Jr., Di Tullio G., Weigert R., Malhotra V., Corda D., De Matteis M. A. and Luini A. (1997). Role of NAD+ and ADP-ribosylation in the mainteinance of the Golgi structure. J. Cell Biol. 139:1109-1118.
Weigert R., Colanzi A., Mironov A., Buccione R., Cericola C., Sciulli M. G., Santini G., Flati S., Fusella A., Donaldson J. G., Di Girolamo M., Corda D., De Matteis M. A. and Luini A. (1997). Characterization of chemical inhibitors of the brefeldin-a-activated mono-adp-ribosylation. J. Biol. Chem. 272: 14200-14207.
Weigert R., Colanzi A., Limina C., Cericola C., Di Tullio G., Mironov A. Luini A. and De Matteis M. A. (1997). Characterization of an endogenous mono ADP-ribosyl transferase stimulated by brefeldin A. Advanced in Experimental Medicine and Biology Vol. 419: 337-342.
Silletta M.G., Di Girolamo M., Fiucci G., Weigert R., Mironov A., De Matteis M. A., Luini A. and Corda D. (1997). Possible role of BARS-50, a substrate of brefeldin A-dependent mono-ADP-ribosylation, in intracellular transport. Advanced in Experimental Medicine and Biology Vol. 419: 321-330.
Colanzi A., Mironov A., Weigert R., Limina C., Flati S., Cericola C., Di Tullio G., Di Girolamo M., Corda D., De Matteis M. A. and Luini A. (1997). Brefeldin A-induced ADP-ribosylation in the structure and function of the Golgi complex. Advanced in Experimental Medicine and Biology 419: 331-335.
Complete CV (PDF Format)
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