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James E. Melvin, D.D.S., Ph.D.

James E. Melvin, D.D.S., Ph.D.Director, Clinical Fellowship Program
Acting Chief Oral and Pharyngeal Cancer Branch
Chief, Secretory Mechanisms and Dysfunctio​n Section

BUILDING 10, ROOM 5-2531 MSC 1470
BETHESDA, MD 20892-4320

Phone:  (301) 402-1706
Fax:  (301) 480-4455

Biographical Sketch

James Melvin, NIDCR Deputy Scientific Director, received his D.D.S. from Case Western Reserve University, a Ph.D. in neurobiology and anatomy from the University of Rochester, and did his postdoctoral training in NIDCR's (then) Clinical Investigation and Patient Care Branch. A former director of the Center for Oral Biology and Professor of Pharmacology and Physiology at the University of Rochester, Dr. Melvin has made numerous contributions to the field of salivary gland physiology and played a key role in the research consortium that decoded the saliva proteome. He directed a number of Ruth L. Kirschstein Institutional National Research Award programs at the pre-doctoral, doctoral and postdoctoral level. 

Research Interests/Scientific Focus

The Secretory Mechanisms and Dysfunction Section investigates the molecular nature and function of the ion transport mechanisms involved in the fluid and electrolyte secretion process in the exocrine salivary gland.  We are probing the structure-function relationships of cotransporter, exchanger and channel proteins using a combination of molecular biology, gene modification, proteomics and functional studies in mouse and human salivary glands.  A high-throughput approach is being used to catalogue the human saliva proteome, to identify salivary biomarkers for human diseases, and to compile a comprehensive list of the plasma membrane proteins expressed in salivary glands.

Selected Publications

  1. Taylor, S.R.J., M. Gonzalez-Begne, S. Dewhurst, G. Chimini, C.F. Higgins, J.E. Melvin, and J.I. Elliott.  2008. Sequential Shrinkage and Swelling Underlie P2X7-Stimulated Lymphocyte Phosphatidylserine Exposure and Death. J. Immunology. 180:300-308. PMID: 18097031
  2. Nakamoto, T., V.G. Romanenko, A. Takahashi, T. Begenisich, and J.E. Melvin. 2008. Apical Maxi-K channels mediate K+ secretion by the mouse submandibular exocrine gland. Am. J. Physiol. Cell. Physiol. 294:C810-C819. PMID: 18216162
  3. Denny, P., F.K. Hagen, M. Hardt, L. Liao, W. Yan, M. Arellano, S. Bassilian, G.S. Bedi, P. Boontheung, D. Cociorva, C.M. Delahunty, T. Denny, J. Dunsmore, K.F. Faull, J. Gilligan, M. Gonzalez-Begne, F. Halgand, S.C. Hall, X. Han, B. Henson, J. Hewel, S. Hu, S. Jeffrey, J. Jiang, J.A. Loo, R.R.O. Loo, D. Malamud, J.E. Melvin, O. Miroshnychen, M. Navazesh, R. Niles, S.K. Park, A. Prakobphol, P. Ramachandran, M. Richert, S. Robinson, M. Sondej, P. Souda, M.A. Sullivan, J. Takashima, S. Than, J. Wang, J.P. Whitelegge, H.E. Witkowska, L. Wolinsky, Y. Xie, T. Xu, W. Yu, J. Ytterberg, D.T. Wong, J.R. Yates III, and S.J. Fisher. 2008. The Proteomes of Human Parotid and Submandibular/Sublingual Gland Salivas Collected as the Ductal Secretions. J. Proteome Res. 7:1994-2006. PMID: 18361515
  4. Toyama, K., H.Wulff, K.G.Chandy, P.Azam, G.Raman, T.Saito, Y.Fujiwara, D.D.Gutterman, D.L.Mattson, S.Das, J.E.Melvin, P.F.Pratt, D.R.Harder, and H. Miura. 2008. Role of the Intermediate-conductance Calcium-activated Potassium Channel, KCa3.1, in Cellular Activation and Atherogenesis. J. Clin. Invest. 118:3025-3037. PMID: 18688283
  5. Romanenko, V.G., T. Nakamoto, M.A. Catalán, M. Gonzalez-Begne, G.J. Schwartz, Y. Jaramillo, F.V. Sepúlveda, C.D. Figueroa, J.E. Melvin. 2008. Clcn2 encodes the hyperpolarization-activated chloride channel in the ducts of mouse salivary glands. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G1058-G1067. PMID: 18801913
  6. Nakamoto, T., D.A. Brown, M. Gonzalez-Begne, V.G. Romanenko, M.A. Catalán, and J.E. Melvin. 2009. Purinergic P2X7 Receptors Mediate ATP-induced Saliva Secretion by the Mouse Submandibular Gland. J. Biol. Chem. 284:4815-4822. PMID: 19097994
  7. Romanenko, V.G., K.S. Roser, J.E. Melvin, and T. Begenisich. 2009. Cholesterol and the Cytoskeleton Control the Interaction of IK1 and Maxi-K Channels. Am. J. Physiol. Cell. Physiol. 296:C878-C888. PMID: 19176762
  8. Gonzalez-Begne, M., B. Lu, X. Han, F.K. Hagen, A.R. Hand, J.E. Melvin, and J.R. Yates, III. 2009. Proteomics Analysis of Human Parotid Gland Exosomes by Multidimensional Protein Identification Technology (MudPIT). J. Proteome Res. 8:1304-1314. PMID: 19199708
  9. Ambatipudi, K.S., B. Lu, F.K. Hagen, J.E. Melvin, and J.R. Yates, III. 2009. Quantitative Analysis of Age Specific Variation in the Abundance of Human Female Parotid Salivary Proteins. J. Proteome Res. 8:5093-5102. PMID: 19764810
  10. Catalán, M.A., T. Nakamoto, M. Gonzalez-Begne, J.M. Camden, S.M. Wall, L.L. Clarke and J.E. Melvin. 2010. Cftr and ENaC Ion Channels Mediate NaCl Absorption in the Mouse Submandibular Gland. J. Physiol. 588:713-724. PMID: 20026617
  11. Romanenko, V.G., M.A. Catalán, D.A. Brown, I. Putzier, H.C. Hartzell, A.D. Marmorstein, C.E. Ovitt, B.D. Harfe and J.E. Melvin. 2010. Tmem16A encodes the Ca2+-activated Cl- channel in Mouse Submandibular Salivary Gland Acinar Cells. J. Biol. Chem. 285:12990-13001. PMID: 20177062
  12. Ambatipudi, K.S., F.K. Hagen, C. Delahunty, X. Han, R. Shafi, J. Hryhorenko, S. Gregoire, R. Marquis, J.E. Melvin, H.M. Koo and J.R. Yates, III. 2010. Human Common Salivary Protein 1 (CSP-1) Promotes Binding of Streptococcus mutans to Experimental Salivary Pellicle and Glucans Formed on Hydroxyapatite Surface. J. Proteome Res. 9:6605-6614. PMID: 20858015
  13. Catalán, M.A., K. Scott-Anne, M.I. Klein, H. Koo, W.H. Bowen and J.E. Melvin. 2011. Elevated Incidence of Dental Caries in a Mouse Model of Cystic Fibrosis. PLoS One. 6:e16549. PMID: 21304986
  14. Gonzalez-Begne, M., B. Lu, L. Liao, T. Xu, C. Salehi, G. Bedi, J.E. Melvin, and J.R. Yates, III. 2011. Characterization of the human submandibular/sublingual saliva glycoproteome using lectin affinity chromatography coupled to Multidimensional Protein Identification Technology. J. Proteome Res. [Epub ahead of print] PMID: 21936497
  15. Catalán, M.A., C.A. Flores, M. González-Begne, Y. Zhang, F.V. Sepúlveda and J.E. Melvin. 2011. Severe Defects in Absorptive Ion Transport in the Distal Colon of Mice Lacking ClC-2 Channels. Gastroenterology (accepted)

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This page last updated: October 07, 2016