I use super-resolution fluorescence microscopy techniques, including Stimulated Emission Depletion (STED) microscopy, to study the localization of GalNAc-Ts in the Golgi compartment and to try and identify a potential localization pattern or a possible hierarchy of GalNAc-Ts that might spatially regulate O-glycosylation. In addition, I am currently developing a Fluorescence Resonance Energy Transfer (FRET) based biosensor that would allow the sensing of spatio-temporal initiation and branching of O-glycosylation in cells, and will be used to understand the regulation of the activity of GalNAc-Ts in cells.
Herbomel G, Grichine A, Fertin A, Delon A, Vourc'h C, Souchier C, Usson Y.
Wavelet transform analysis of chromatin texture changes during heat shock. J Microsc. 2015 Dec 22.
Herbomel G, Kloster-Landsberg M, Folco EG, Col E, Usson Y, Vourc'h C, Delon A, Souchier C.
Dynamics of the full length and mutated heat shock factor 1 in human cells. PLoS One. 2013 Jul 8;8(7):e67566
Kloster-Landsberg M, Herbomel G, Wang I, Derouard J, Vourc'h C, Usson Y, Souchier C, Delon A.
Cellular response to heat shock studied by multiconfocal fluorescence correlation spectroscopy.
Biophys J. 2012 Sep 19;103(6):1110-9