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Rapid Test for Sjögren’s Syndrome

October 21, 2009

schematic of antibodiesA team of NIDCR scientists reported in August that an experimental laboratory test called LIPS had correctly identified a key antibody associated with primary Sjögren’s syndrome three out of four times and with perfect accuracy.   These data represented a significant improvement over the sensitivity and specificity of today’s standard ELISA assays.  Now, the scientists report in the October issue of the Journal of Translational Medicine that this two-hour test just got a whole lot quicker.  The scientists streamlined the assay’s two one-hour incubation steps to five minutes each.  As the author’s reported, their quicker technique called QLIPS “includes a 5 minute set-up, two five-minute incubation steps, 10 minutes of washing and reading of the [testing] plate with a luminometer” for the results.  Total testing time:  About 25 minutes.  Today’s ELISA tests typically range from five hours to a day to complete. 

In the current article, the authors evaluated the ability of QLIPS to detect autoantibodies against three proteins - La, Ro60, and Ro52 – that are often associated with primary Sjögren’s syndrome.  They found that QLIPS detected the autoantibodies with a sensitivity of 49 percent for La, 56 percent for Ro60, and 70 percent for Ro52.  The latter result was particularly noteworthy because it was a better diagnostic outcome than the longer-incubating LIPS technique.  Moreover, QLIPS detected antibody titers from Ro52-positive Sjögren’s patients that were 1,000 times higher than those in healthy volunteers.  Taken together, these results suggest that QLIPS could measure Ro52 levels alone to diagnose primary Sjögren’s syndrome.  The data also suggest that QLIPS, with its speed and automated ease, has great potential as a point-of-care diagnostic test for Sjögren’s and other autoimmune disorders.



 

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This page last updated: February 26, 2014