Antisera

Colleague:

I am pleased that you are interested in using the antisera and/or cDNA probes that I make available to researchers. Please check the cross-reactivity panel for antisera on the Reagents page to see if your choice of antisera is appropriate for your model system. In order to reduce the legal and shipping costs for the NIH and to reduce the time required for colleagues at non-profit research institutions to obtain the reagents, the NIH has requested that the most commonly requested antisera be listed, aliquoted and shipped by KeraFast (see list below), EMD Millipore Corp. (for LF-104, LF-110, LF-112, LF-114 & LF-136) or Santa Cruz Biotechnology (for all monoclonal antisera).

 

Antibodies for KeraFast

 LF-39  Human amino propeptide of alpha 1 chain of type I collagen
 LF-69  Human Type III collagen carboxy-propeptide
 LF-71  Human Type III collagen amino-propeptide
 LF-84  Human Bone Sialoprotein
 LF-94  Bovine Decorin
 LF-96  Bovine Biglycan
 LF-99  Human Versican
 LF-100  Human Bone Sialoprotein
 LF-100  Human Bone Sialoprotein
 LF-120  Human Bone Sialoprotein
 LF-122  Human Decorin
 LF-126  Human Osteocalcin
 LF-148  Human DMP1
 LF-150  Mouse Fibromodulin
 LF-151  Human DSPP (DSP domain)
 LF-153  Mouse DSPP (DSP domain)
 LF-154  Human DSPP (DPP domain)
 LF-155  Human MEPE
 LF-160  Human DMP1
 LF-161  Human Osteopontin (Exon 4)
 LF-166  Human Osteopontin
 LF-175  Mouse Osteopontin
 LF-182  Human MMP-3
 LF-183  Human MMP2
 LF-184  Human MMP-9
 LF-190  Mouse DSPP (DPP domain)

 

For most monoclonals, animal species other than human, monkey & mouse have not been tested.

To comply with the National Institutes of Health’s requirements I will generate a Transfer Memo using the information you send to me.  After the NIH has assigned a tracking number to the request document I will forward it to you.  You will complete the document and email the completed form to NIDCReMTA@mail.nih.gov. Contact Dr. David Bradley, bradleyda@nidcr.nih.gov, if you have any questions about the official form.  (I can usually answer questions about the reagents themselves, but NIH controls the procedures about releasing them to you.)

When sending your initial information to me, please note a very brief research project (1-3 sentences) including the model species (mouse, cow, human etc.) and techniques you plan to use (paraffin sections, Western blot etc.) so that I can send the most appropriate antisera/cDNA. I will send the Research Materials after NIH has accepted the completed requests and instructed me to send the materials. I reserve the right to limit the number probes sent (particularly in the case of duplicate requests of antisera for detecting the same protein) and to substitute in cases where antisera are limited or I think that a different probe would be more effective. Please remember that our web site is not often updated. My apologies for any errors or confusion.

For colleagues in COMMERCIAL laboratories, you MUST contact the Technology Development Coordinator here at the NIDCR (Dr. David Bradley, bradleyda@nidcr.nih.gov) about the necessity of obtaining a license for the probes. Questions about legal or technical aspects of the SLA should be directed to Dr. Bradley.

Upon receipt of the signed SLA from NIH, I will ship without charge by regular airmail. For requests from within the USA, inclusion of your FedEx number (and phone number) may shorten the shipping times at your expense.

Sincerely,

Larry W. Fisher, PhD
Chief, Matrix Biochemistry Section
Craniofacial and Skeletal Diseases Branch
NIDCR/NIH

 

The list of Antisera are shown in the following tables:

 

Proteoglycan polyclonal antisera

Gene product Antiserum Name Antigen source Known species Comments References
Human decorin LF-136 GIGPEVPDDRDF-[KLH] Hum & Mon only Increased signal strength with pretreatment with ABC'ase

Fisher et al. 1989b

Bianco et al. 1990

Human decorin propeptide LF-110 QVSWAGPFQQRGLFDC-[LPH] Hum, others not determined but seq No cross to BGN propeptide Fisher et al.
1995
Human decorin propeptide LF-111 QVSWAGPFQQRGLFDC-[CSA] (amino acids 5-17) Hum, others not determined but seq is highly conserved No cross to BGN propeptide Fisher et al.
1995
Human decorin LF-122 recombinant protein including propeptide Hum, Mon, Pig, She, Chi, Cow (?)   Fisher et al.
1995
Human biglycan LF-51 GVLDPDSVTPTYSA-[BSA] (amino acids 11-24) same conjugate for all three antisera Hum & Mon only Increased signal strength with pretreatment with ABC'ase

Fisher et al. 1989b

Bianco et al. 1990

Human biglycan LF-112 GVLDPDSVTPTYSA-[BSA] (amino acids 11-24) same conjugate for all three antisera Hum & Mon only Increased signal strength with pretreatment with ABC'ase

Fisher et al. 1989b

Bianco et al. 1990

Human biglycan propeptide LF-104 LPFEQRGFWDFTLDDC-[LPH] Hum, Mon, Rat, Mou   Fisher et al. 1995
Human biglycan propeptide LF-105 LPFEQRGFWDFTLDDC-[CSA] Others not determined   Fisher et al.
1995
Human veriscan LF-99 LHKVGKSPPVRC-[LPH] Only human tested   Fisher et al. 1995
Human veriscan LF-98 LHKVGKSPPVRC-[CSA]      
Human biglycan LF-121 recombinant protein including propeptide Hum & Mon only   Fisher et al. 1995
Bovine decorin LF-94 IGPEEHFPEVPEC-[LPH] Only Cow tested Cow domain analogous to Human LF-30 Fisher et al. 1995
Bovine decorin LF-95 IGPEEHFPEVPEC-[CSA] For these 2 antisera Cow domain analogous to Human LF-30 Fisher et al. 1995
Bovine biglycan LF-96 LPDLDSLPPTYSC-[LPH] Only Cow tested Cow domain analogous to Human LF-15 series Fisher et al. 1995
Bovine biglycan LF-97 LPDLDSLPPTYSC-[CSA] For these 2 antisera Cow domain analogous to Human LF-15 series Fisher et al. 1995
Murine decorin LF-113 IIPYDPDNPLISMC-[LPH] Only tested for Mouse Mouse domain analogous to Human LF-30 series Fisher et al.
1995
Murine decorin LF-114 IIPYDPDNPLISMC-[CSA] Mou and Rat Mouse domain analogous to Human LF-30 series Fisher et al.
1995
Murine biglycan LF-106 VPDLDSVTPTFSAMS-[LPH] Only tested for Mouse Mouse domain analogous to Human LF-15 series Fisher et al.
1995
Murine biglycan LF-107 VPDLDSVTPTFSAMS-[CSA] Mou and Rat Mouse domain analogous to Human LF-15 series Fisher et al.
1995
Murine biglycan LF-159 VPDLDSVTPTFSAMS-(LPH) Mou and Rat Mouse domain analogous to Human LF-15 series  
Murine fibromodulin LF-149 CDKVGRKVFSKLRHLER & CDPYDPYPYEPSEPYPYGVEE conjugated to KLH Mouse, others not tested
Histology requires digestion with chondrointinase  ABC
 
Murine fibromodulin LF-150 CDKVGRKVFSKLRHLER & CDPYDPYPYEPSEPYPYGVEE conjugated to KLH Mouse, others not tested    

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Sialoprotein polyclonal antisera

Gene Product Antiserum Name Antigen Source Known Species Cross-reactivity Comments References
Human osteopontin LF-19 IPVKQADSGE homopolymer Hum & Mon only   Fisher et al. 1989b
Human osteopontin LF-86 TVDTYDGRGDSVVYGLR
SLS-[LPH]
Hum, Mon human osteopontin RGD domain unknown utility Fisher et al. 1995
Human osteopontin LF-161 CLYNKYPDAVATWLNPDPSQKQNLLAPN conjugated to KLH only human tested human exon 4  
Human osteopontin LF-166 recomb. Carboxyl half starts at thrombin site Hum, Mon, Pig, Cow, She, Rat, Mou does not contain the RGD domain Fisher et al. 1995 based on OP-10 plasmid (Young et al. 1990a)
Human osteopontin LF-124 recombinant amino half ending at thrombin site Hum, Mon, Cow, She, Rat, Mou contains the RGD domain Fisher et al. 1995 based on OP-10 plasmid (Young et al. 1990a)
Mouse OPN LF-175 recombinant mouse OPN (E coli) Mou, Rat OK for WB, IF, IHC(P)  
Human osteopontin LFMb-14 Monoclonal*, IgG2b human osteopontin, likely epitope is within KFRRPDIQYPDATD All tested to date: Hum, Mou, Rat, Sheep, Rabbit, Monkey Mouse monoclonal (IgG) works in westerns and paraffin sections  
Human bone sialoprotein LFMb-24 Monoclonal*, IgG1 recombinant (AA131-442) Hum, Monkey May be slightly more sensitive on WB than LFMb-25 Bellahcène A, Castronovo V, Ogbureke KU, Fisher LW, Fedarko NS.  Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.
Human bone sialoprotein LFMb-25 Monoclonal*, IgG1 recombinant (AA131-442), epitope probably within: VAYEGEYEYTGANGYDNGYE Hum, Monkey OK for WB, IF & IHC(P) Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70.  Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.
Human bone sialoprotein LF-83 YESENGEPRGDNYRAYE
D-[LPH]
Hum, Mon, Dog epitope appears to be RAYED Bianco et al. 1993
Mintz et al. 1993
Human bone sialoprotein LF-84 YESENGEPRGDNYRAYE
D-[LPH]
Rat, Mou, Chi   Fisher et al. 1995
Human bone sialoprotein LF-100 AIQLPKKAGDIC-[LPH] Hum, Mon, Dog, Rat mid-protein antigen Mintz et al. 1993
Human bone sialoprotein LF-101 AIQLPKKAGDIC-[CSA] Hum, Mon, Dog, Rat, Pig, Cow mid-protein antigen Fisher et al. 1995
Human bone sialoprotein LF-119 recombinant RGD domain (AA257-317) Hum, Mon, Dog, Chi mid-protein antigen similar to LF-100 Fisher et al. 1995 based on B6-5g plasmid (Fisher et al. 1990)
Human bone sialoprotein LF-120 recombinant Frag 1 (aa 129-281) Hum, Mon, Pig, Dog, Rat   Fisher et al. 1995 based on B6-5g plasmid (Fisher et al. 1990)
Rat (UMR) bone sialoprotein LF-87 rat BSP isolated from UMR-106-BSP media Rat, Mou, Hom full sulfated form of rat BSP Fisher et al. 1995
Rat (UMR) bone sialoprotein LF-90 rat UMR-106 BSP grown in chlorate Rat Low sulfate(<5%) sulfate 0n BSP Fisher et al. 1995
Human dentin matrix protein-1 (DMP1) LFMb-31 recombinant exon 5 (last), epitiope probably within: SESQEEVVSESRGDNPDPTTSYVE Hum, Monkey OK for WB (but DMP1 is difficult to capture in standard WB) IF, IHC(P) Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70.  Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.
Human dentin matrix protein 1 (DMP1) LF-148 CEHPSRKIFRKSRISE and CLKNIEIESRKLTVDAYH conjugated to KLH human and bovine, others not tested    
Human dentin matrix protein 1 (DMP1) LF-160 CGSDDHQYIYRLAGGFSRSTGKGGDD & CRDTGLSQPRRDSKGDSQED conjugated to KLH human and bovine, others not tested    
Human DSP (of DSPP) LFMb-21 Monoclonal*, IgG1 synthetic peptide: KGQEGQDHGKEDDHDS Hum, Monkey OK for WB, IF, IHC(P) Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70.  Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.
Hum dentin sialophosphoprotein (DSPP) LF-151 part of DSP portion of human DSPP, bacterial recombinant human and bovine, not mouse    
Hum dentin sialophosphoprotein (DSPP) LF-154 non repetitive portion of last exon, human DSPP human and mouse, others not tested appears to recognize DSP portion of last exon  
Mou dentin sialophosphoprotein (DSPP) LF-153 part of DSP portion of mouse DSPP, bacterial recombinant mouse, not human or cow    
Human MEPE LFMb-31 Monoclona*, IgG1 recombinant last exon, epitope within THRIQHNIDYLKHLSKVKKI Hum, Monkey OK for WB, IF, IHC(P) Ogbureke KU, Fisher LW. Expression of SIBLINGs and their partner MMPs in salivary glands. J Dent Res. 2004 Sep;83(9):664-70.  Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): multifunctional proteins in cancer. Nat Rev Cancer. 2008 Mar;8(3):212-26.
Human MEPE LF-155 last exon of human MEPE made in bacteria human, others not tested    
Human MEPE LF-158 CSIYPKSTGNKGFEDGDD & CSTHRIQHNIDYLKHLSKVKK conjugated to KHH human, others not tested weaker titer than LF-155 on ELISA  

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Collagen polyclonal antisera

Gene Product Antiserum Name Antigen Source Known Species Cross-reactivity Comments References
Bovine a1 (I) amino-propepetide LF-9 bovine bone N-propeptide Hum, Mon, Pig, She   Fisher et al. 1987b
Human a1 (I) amino-propepetide LF-39 EEGQVEGQDEDIPPITCVQ-[KLH] Hum, Cow, She   Fisher et al. 1989a
Human a1 (I) amino-propepetide LF-40 EEGQVEGQDEDIPPITCVQ-[KLH] Hum, Cow, She   Fisher et al. 1989a
Human a1 (I) carboxy-propept. LF-41 PLDVGAPDQEFGFDVGPVCFL-[KLH] All species except, perhaps Rab detects proal(1) and C-propeptide Fisher et al. 1989a
Human a1 (I) carboxy-propept. LF-42 PLDVGAPDQEFGFDVGPVCFL-[KLH]     Fisher et al. 1995
Human a1 (I) carboxy-telopept. LF-67 SAGFDFSFLPQPPQEKAHDGGRYYRA All species except, detects proal(1) Bernstein et al. 1995b
Human a1 (I) carboxy-telopept. LF-68 SAGFDFSFLPQPPQEKAHDGGRYYRA perhaps Rab pCal(1), a(1) + / - reduction Fisher et al. 1995
Human a2 (I) amino-telopeptide LF-116 QYDGKGVGLGPGPC-[LPH] Hum, not mouse, others not tested    
Human type III collagen C-propeptide LF-69 DIGGPDQEFGVDVGPVCFL Hum, Mon, others not tested seq. homology to type II but not tested. Reduction increases reaction Bernstein et al. 1995b
Human type III collagen C-propeptide LF-70 DIGGPDQEFGVDVGPVCFL Hum, Mon, others not tested seq. homology to type II but not tested. Reduction increases reaction Fisher et al.
1995
Human type III collagen N-telopeptide LF-71 EYDSYDVKSGVAC-[KLH] Hum, Mon   Bernstein et al. 1995b
Human type III collagen N-telopeptide LF-72 EYDSYDVKSGVAC-[KLH] others not tested   Fisher et al.
1995

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Miscellaneous polyclonal antisera

Gene Product Antiserum Name Antigen Source Known Species Cross-reactivity Comments References
Human osteonectin LF-37 EALPDETEVVEETVAEVT
EVP-[KLH] (AA 4-25)
Hum, Mon Also known as SPARC Fisher et al. 1995
Bovine osteonectin BON-I
BON-II
bovine bone osteonectin (both) All species tested Also known as SPARC Ingram et al. 1993
Bovine osteonectin LF-55
LF-56
first 56 AA of mature Cow sequence (both) only Cow and Hum tested Also known as SPARC Fisher et al. 1995
Bovine osteonectin LF-53
LF-54
AA 27-56 of mature Cow sequence (both) only Cow and Hum tested Also known as SPARC Fisher et al. 1995
Chicken osteonectin LF-8 chicken bone osteonectin Chi, Quail Also known as SPARC Fisher et al. 1995
Chicken osteonectin LF-45 APQEALPDETEVIED-[KLH] Chi, Quail AA 1-15 of mature Fisher et al. 1995
Mouse osteonectin LF-23
LF-24
TEVAEEIVE EET-[KLH] (both) (AA 5-16 of mature) Mou, Rat, She, Chi Also known as SPARC Wewer et al. 1988
Bovine osteocalcin LF-32 bovine bone osteocalcin Cow, Hum, Mon also known as bone gla protein (BGP) Ingram et al. 1993
Bovine alkaline phosphatase LF-52 from bovine kidney affinity purified by Sigma unknown bone-liver-kidney form of the enzyme Fisher et al. 1995
Human alkaline phosphatase LF-47
LF-48
LVPEKEKDPKYWRDQAQ
ETC-[KLH] (both) (AA 1-19)
Hum (weak), Cow, Pig, Rab human sequence but weak activity Tulli et al. 1992
Human osteoinductive factor (OIF) LF-102
LF-103
CLKRLPIGSYF-(LPH)
CLKRLPIGSYF-(CSA)
both not tested C-term of OIF, both uncharacterized Fisher et al. 1995
Human MMP-3 LF-182 synthetic peptide: EPGFPKQIAEDFPGIDSKIDAC Hum, Monkey IHC(P), WB probably OK Ogbureke KU, Fisher LW.
SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem.
  2007 Apr;55(4):403-9. Epub 2007
Jan 8.
Human MMP-2 LF-183 synthetic peptide: ENQSLKSVKFGSIKSDWLGC Hum, Monkey IHC(P), WB probably OK Ogbureke KU, Fisher LW.
SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem. 
2007 Apr;55(4):403-9. Epub 2007 
Jan 8.
Human MMP-9 LF-184 synthetic peptide: RSELNQVDQVGYVTYDILQCPED Hum, Monkey IHC(P), WB probably OK Ogbureke KU, Fisher LW.
SIBLING expression patterns in duct epithelia reflect the degree of metabolic activity. J Histochem Cytochem. 
2007 Apr;55(4):403-9. Epub 2007 
Jan 8.
Mouse MMP-14 LF-176 catalytic domain synthetic peptide: CPLRFREVPYAYIREGHEKQAD Hum, Mouse IHC, WB  
Mouse MMP-14 LF-177 hemopex domain synthetic peptide: CRTTSRPSVPDKPKNPAYG Hum, Mouse IHC(P), WB  
Mouse MMP-14 LF-178 combined peptides from LF-176 & LF-177 Hum, Mouse IHC(P), WB  

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Last Reviewed
July 2018